Novel insights in dimethyl carbonate-based extraction of polyhydroxybutyrate (PHB)
نویسندگان
چکیده
منابع مشابه
Cyanobacterial Polyhydroxybutyrate (PHB): Screening, Optimization and Characterization
In modern life petroleum-based plastic has become indispensable due to its frequent use as an easily available and a low cost packaging and moulding material. However, its rapidly growing use is causing aquatic and terrestrial pollution. Under these circumstances, research and development for biodegradable plastic (bioplastics) is inevitable. Polyhydroxybutyrate (PHB), a type of microbial polye...
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Polyhydroxybutyrate [P(3HB)] was produced in the transgenic tobacco harboring the genes encoding acetoacetyl-CoA reductase (PhaB) and polyhydroxyalkanoate synthase (PhaC) from Ralstonia eutropha (Cupriavidus necator) with optimized codon usage for expression in tobacco. P(3HB) contents in the transformants (0.2mg/g dry cell weight in average) harboring the codon-optimized phaB gene was twofold ...
متن کاملA Closer Look on the Polyhydroxybutyrate- (PHB-) Negative Phenotype of Ralstonia eutropha PHB-4
The undefined poly(3-hydroxybutyrate)- (PHB-) negative mutant R. eutropha PHB-4 was generated in 1970 by 1-nitroso-3-nitro-1-methylguanidine (NMG) treatment. Although being scientific relevant, its genotype remained unknown since its isolation except a recent first investigation. In this study, the mutation causing the PHA-negative phenotype of R. eutropha PHB-4 was confirmed independently: seq...
متن کاملComparative proteome analysis reveals four novel polyhydroxybutyrate (PHB) granule-associated proteins in Ralstonia eutropha H16.
Identification of proteins that were present in a polyhydroxybutyrate (PHB) granule fraction isolated from Ralstonia eutropha but absent in the soluble, membrane, and membrane-associated fractions revealed the presence of only 12 polypeptides with PHB-specific locations plus 4 previously known PHB-associated proteins with multiple locations. None of the previously postulated PHB depolymerase is...
متن کاملStudies on PHB (Polyhydroxybutyrate) degradation by some species of Aspergillus
PHB degradation capabilities of seven species of Aspergillus namely Aspergillus niger, Aspergillus flavus, Aspergillus ochraceus, Aspergillus nidulans, Aspergillus terreus, Aspergillus fumigatus and Aspergillus parasiticus on the polymer produced by Rhodospseudomonas palustris KU003 were assayed . Among all the fungi investigated, the ability of A.niger to utilize the biopolymer was the highest...
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ژورنال
عنوان ژورنال: Biotechnology for Biofuels
سال: 2021
ISSN: 1754-6834
DOI: 10.1186/s13068-020-01849-y